Journal: Experimental & Molecular Medicine

Article Title: Altered AKAP12 expression in portal fibroblasts and liver sinusoids mediates transition from hepatic fibrogenesis to fibrosis resolution

doi: 10.1038/s12276-018-0074-5

Figure Lengend Snippet: a – c Liver sections from WT or AKAP12 KO mice fed a normal diet (ND), DDC-containing diet for 3 weeks (DDC), and DDC-containing diet for 3 weeks followed by a normal diet for 2 weeks (RES), which were stained with Sirius Red ( a ) and IHC against collagen I ( b ), and collagen III ( c ). Scale bars indicate 500 µm. Graphs are morphometric quantifications of areas stained by Sirius Red, collagen I, and collagen III. Data are expressed as the mean ± SEM. n = 4 mice for ND, n = 10 mice for DDC, n = 11–12 mice for RES ( a ). n = 2 mice for ND, n = 5 mice for DDC, n = 5–6 mice for RES ( b , c ). d Relative mRNA levels of Col1a1, Fibulin1, and Elastin in liver tissues were quantified by qPCR with specific primers. n = 2 mice for ND, n = 5 mice for DDC, n = 5 mice for RES. Data are expressed as the mean ± SEM. e , f Liver sections were subjected to IHC to detect laminin and desmin ( e ) or VEGFR2 and F4/80 ( f ). Scale bars indicate 100 µm. Graphs show relative areas positive for each type of staining. Data are expressed as the mean ± SEM. n = 2 mice for ND, n = 5 mice for DDC, n = 5–6 mice for RES. * P < 0.05; ** P < 0.01; *** P < 0.001; NS not significant

Article Snippet: After blocking with 5% normal donkey serum (Sigma-Aldrich) in PBS solution, the sections were incubated overnight at 4 °C with primary antibodies for AKAP12 (I. Gelman, Roswell Park Cancer Institute), aSMA (Dako), laminin (Sigma), SE-1 (R&D Systems), CD31 (BD Biosciences), Thy-1 (BD Biosciences), collagen I (Southern Biotech), collagen III (Southern Biotech), Desmin (R&D Systems), VEGFR2 (Cell Signaling), F4/80 (Abcam), SE-1 (R&D Systems), and collagen IV (Southern Biotech).

Techniques: Staining

Journal: Regenerative Therapy

Article Title: miR-145-enriched BMSCs-derived exosomes ameliorate neurogenic erectile dysfunction in aged rats via TGFBR2 inhibition

doi: 10.1016/j.reth.2025.04.004

Figure Lengend Snippet: Characterization of CCSMCs and their interaction with exosomes . (A) Primary CCSMCs migrating from cavernous tissue explants. (B) Immunofluorescence staining with anti-α-SMA and anti-Desmin antibodies confirming the identity of CCSMCs. (C) Internalization of PKH67-labeled Exo and Exo-145 by CCSMCs, showing green fluorescence within the cytoplasm. (D) Elevated miR-145 expression levels in CCSMCs after co-culture with Exo or Exo-145. Data are presented as mean ± SEM. ∗∗∗ p < 0.001, ns: no significant difference.

Article Snippet: Third-generation CCSMCs were identified through immunofluorescence staining for desmin (1:200, 16520-1-AP, Proteintech) and α-smooth muscle actin (1:200, 14395-1-AP, Proteintech).

Techniques: Immunofluorescence, Staining, Labeling, Fluorescence, Expressing, Co-Culture Assay

Journal: Human molecular genetics

Article Title: Expression of an LMNA-N195K variant of A-type lamins results in cardiac conduction defects and death in mice.

doi: 10.1093/hmg/ddi221

Figure Lengend Snippet: Figure 5. Increased interstitial fibrosis was observed in LmnaN195K/N195K heart tissue. Sections of ventricular tissue from a WT (A) and mutant (B) heart were stained with Masson’s trichrome for collagen. Magnification is the same for the two panels. (C and D) Desmin localization in hearts of WT (C) and LmnaN195K/N195K animals (D). Desmin staining appears green and tissue was counterstained blue with DAPI to visualize nuclei.

Article Snippet: Additional primary antibodies were as follows: a-connexin40, a-connexin43 from Zymed (South San Francisco, CA, USA) and a-HF1b/SP4, a-actin and a-desmin from Santa Cruz.

Techniques: Mutagenesis, Staining

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: Disassembly of Myofibrils and Potential Imbalanced Forces on Z-Discs in Cultured Adult Cardiomyocytes

doi: 10.1002/cm.21298

Figure Lengend Snippet: (A) The patterns of desmin in the different shapes of cardiomyocytes. The striated pattern was still visible at the middle region (red arrows), but disappeared at the ends of a peanut-shape cell (yellow arrows). (B) Striated myofibril structure is clearly shown in a LRS cardiomyocyte, and the patterns of desmin and α-actinin are co-localized. (C) The striated myofibril patterns disappeared at the cell ends (yellow arrows) and the patterns of desmin and α-actinin became mismatched in peanut-shape cells. At the middle region of the cell (red arrows), the striated patterns of desmin and α-actinin are still visible and co-localized. Scale bar: 10 μm.

Article Snippet: After permeabilizing the cells using Triton X-100 and blocking the active sites in blocking solution (0.2% Triton X-100, 5% normal serum and 1% bovine serum albumin in phosphate buffered saline) for 30 min at room temperature, primary antibodies, including anti-α-actinin (Abcam), anti-connexin-43 (Santa Cruz), anti-MyHC (Abcam), anti-plakoglobin, and anti-desmin (Novus Biologicals) were applied.

Techniques:

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: Disassembly of Myofibrils and Potential Imbalanced Forces on Z-Discs in Cultured Adult Cardiomyocytes

doi: 10.1002/cm.21298

Figure Lengend Snippet: (A) Representative images of cardiomyocytes cultured in medium with or without 25 μM BLB at different time points (Day 0–4). Scale bar: 100 μm. (B) The percentage of LRS cardiomyocytes. *, P < 0.05 versus Control. (C-D) BLB maintained the distribution of α-actinin and preserved desmin and myofibril structure. Scale bar: 50 μm. CTRL: control, BLB: Blebbistatin.

Article Snippet: After permeabilizing the cells using Triton X-100 and blocking the active sites in blocking solution (0.2% Triton X-100, 5% normal serum and 1% bovine serum albumin in phosphate buffered saline) for 30 min at room temperature, primary antibodies, including anti-α-actinin (Abcam), anti-connexin-43 (Santa Cruz), anti-MyHC (Abcam), anti-plakoglobin, and anti-desmin (Novus Biologicals) were applied.

Techniques: Cell Culture

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: Disassembly of Myofibrils and Potential Imbalanced Forces on Z-Discs in Cultured Adult Cardiomyocytes

doi: 10.1002/cm.21298

Figure Lengend Snippet: (A-C) Freshly isolated cardiomyocytes connected through intercalated discs. (A) and (B) Cardiomyocytes with well-preserved intercalated discs. (C) Cardiomyocytes with damaged intercalated discs. The local movement in the cardiomyocytes was analyzed using contractile image analysis. (A-B) Contraction wave (white rectangle in A) in both cardiomyocytes was well-synchronized at the intercalated discs (white arrows). Similar local displacement fields at each side of well-preserved intercalated discs (B). Completely different local displacement fields at each side of damaged intercalated discs (C). (D-E) The cardiomyocytes (Day 1) were double stained with α-actinin (green) and connexin 43 (C×43) (red) (D), desmin (green) and plakoglobin (red) (E), respectively. Blue: nucleus. Yellow arrows indicate disassembly of myofibrils at the cell ends. Scale bar: 50 μm.

Article Snippet: After permeabilizing the cells using Triton X-100 and blocking the active sites in blocking solution (0.2% Triton X-100, 5% normal serum and 1% bovine serum albumin in phosphate buffered saline) for 30 min at room temperature, primary antibodies, including anti-α-actinin (Abcam), anti-connexin-43 (Santa Cruz), anti-MyHC (Abcam), anti-plakoglobin, and anti-desmin (Novus Biologicals) were applied.

Techniques: Isolation, Staining

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Mean percentages of rabbit MSCs that were labeled with each of the 25 markers, as determined by flow cytometry.

Article Snippet: Secondary antibodies were used following the staining of human MSCs with mouse anti-human CD14, CD44, CD45, CD90, CD166, MHC I, MHC II (all from BD PharMingen, San Diego, CA), desmin (GeneTex), and α-SMA (AbD Serotec Ltd.) monoclonal antibodies; the rat anti-human CD34 (GeneTex) monoclonal antibody; and the rabbit anti-human CD106 (Bioss Inc.) polyclonal antibody.

Techniques: Labeling, Cytometry

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Mean percentages of human MSCs that are labeled with each of the 25 markers, as determined by flow cytometry.

Article Snippet: Secondary antibodies were used following the staining of human MSCs with mouse anti-human CD14, CD44, CD45, CD90, CD166, MHC I, MHC II (all from BD PharMingen, San Diego, CA), desmin (GeneTex), and α-SMA (AbD Serotec Ltd.) monoclonal antibodies; the rat anti-human CD34 (GeneTex) monoclonal antibody; and the rabbit anti-human CD106 (Bioss Inc.) polyclonal antibody.

Techniques: Labeling, Cytometry

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Mean percentages of rabbit adipose derived stem cells that were labeled with each of the 25 markers, as determined by flow cytometry.

Article Snippet: Secondary antibodies were used following the staining of human MSCs with mouse anti-human CD14, CD44, CD45, CD90, CD166, MHC I, MHC II (all from BD PharMingen, San Diego, CA), desmin (GeneTex), and α-SMA (AbD Serotec Ltd.) monoclonal antibodies; the rat anti-human CD34 (GeneTex) monoclonal antibody; and the rabbit anti-human CD106 (Bioss Inc.) polyclonal antibody.

Techniques: Derivative Assay, Labeling, Cytometry

Journal: PLoS ONE

Article Title: Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0111390

Figure Lengend Snippet: Comparison of data from the current study with data from four previous studies that examined the phenotypes of human and rabbit MSCs.

Article Snippet: Secondary antibodies were used following the staining of human MSCs with mouse anti-human CD14, CD44, CD45, CD90, CD166, MHC I, MHC II (all from BD PharMingen, San Diego, CA), desmin (GeneTex), and α-SMA (AbD Serotec Ltd.) monoclonal antibodies; the rat anti-human CD34 (GeneTex) monoclonal antibody; and the rabbit anti-human CD106 (Bioss Inc.) polyclonal antibody.

Techniques: Marker, Expressing